In order to search for anti-influenza virus type A agents from Korean traditional prescriptions (herp complexes), we selected 63 traditional prescriptions, based on a review of the Korean trad-itional medicine books. Both methanol extracts and
boiling-water extracts were tested, by means of the Haemagglutination Inhibition Test (HTT).
Three of the 63 methanol extracts: CM-22, CM-26, CM-48 (see explanation of nomenclature below), showed efficacy against influenza virus type A. CM-22 showed anti-influenza virus type A activity at the range of 313 g/ml to 9.75 g/ml, CM-26 showed
antiviral activity at the range of 156 l/ml to 4.87 g/ml, CM-48 showed anti-influenza virus type A activity at the range of 625 g/ml to 19.5 g/ml, respectively. Three of the water extracts:CW-14, CW-34, CW-61 were active. CW-14 showed
anti-influenza
virus type A activity at the range of 10mg/ml to 78 g/ml, CW-34 showed antiviral activity at the range of 10mg/ml to 625 g/ml and CW-61 showed anti-influenza virus type A activity at the range of 10mg/ml to 313 g/ml, respectively. In order to
determine
cytotoxicity of each extracts, chicken rad blood cells were incubated with the various concentration of extracts of Korean traditional prescription. CW-14, CW-34 and CW-61 did not show cytotoxic effect against red blood cells whereas CM-22, CM-26
and
CM-48 showed cytotoxic effect against red blood cells at the range of 10mg/ml to 625 g/ml, 10mg/ml to 313 g/ml and 10mg/ml to 313 g/ml, respectively. These results indicated that Korean traditional prescriptions may be inhibit either attachment
of
virus
to cell surface receptor or penetration of the virus into cell during the initial stage of infection.
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